Izvestiya of Saratov University.
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

real-time PCR

Differentiation of Yersinia pestis Strains of the Main Subspecies of Medieval Biovar by Real-Time PCR Method with Hybridization-Fluorescenсe Registration and SNP Typing

Methods of differentiation and identification of Yersinia pestis strains of medieval biovar by real-time PCR method with hybridization-fluorescent results’ registration and SNP typing method have been developed. Ninety-one Y. pestis strains of medieval biovar from the plague foci of Russia and other CIS countries, received from the State Collection of Pathogenic Bacteria at the Russian Research Anti-Plague Institute “Microbe”, were used in this work. The strains of medieval biovar are represented by four already known phylogenetic lines: 2.MED0, 2.MED1, 2.MED2, 2.MED3.