The article presents the results of testing and optimization of the sample preparation technique for fl ow cytometric analysis of two species that have not been previously studied in this aspect: Colchicum bulbocodium subsp. versicolor and C. laetum. We are using optimal LB01 buff er with added sodium thiosulfate, as internal standards we take Petroselinum crispum and Secale cereale. For the fi rst time, the DNA content in living samples of C. bulbocodium subsp. versicolor (2C = 8.049 pg) and C.
