The optimum variant of explant sterilization of mountain ash var. «Granatnaya» for introduction in culture in vitro has been picked up. It is shown that the nutrient medium WPM with addition of 1,0 mg/l of BAP is optimum for klonal mikropropogation, spontaneous regenerant rooting being found only on nutrient media without hormones. It has been established that the optimum variant of the nutrient medium at the stage of rooting is 1/2WPM with addition of 1,0 mg/l of NАА.