In the article was given the mathematical approaches to classification and clustering of data of acoustic signals in communication between myrmecophilic beetles (Scydmaenus hellwigii) and ants (Formica rufa). For the first time for these species were used a cluster analysis which revealed four clusters, each of them has its own command from the beetle to the ant. Each command was defined by specific ranges of dominant frequencies and a range of medium amplitudes (the values of the parameters R).
