The formation process of quantum dots stabilized by the trioctylphosphine oxide and arachidic acid molecules mixed monolayers was studied. An equation that determines a dependence of numbers of organic matrix molecules that separates quantum dots on e from each other in the close-packing Langmuir monolayer from quantum dots size was proposed. An existence of minimal numbers of organic matrix molecules for changing and tuning a distance between quantu m dots in the Langmuir monolayer was shown.