Izvestiya of Saratov University.
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

gradient and isocratic modes

Optimization of the Conditions for Determining 2,4-D and Deltamethrin by HPLC

The paper presents the results of optimizing the conditions for the chromatographic determination of 2,4-dichlorophenoxyacetic acid and deltamethrin for two capillary columns (Supelcosil LC-18, Kromasil C-18). The modes of individual chromatography of pesticides were selected. The use of the isocratic elution mode in the joint presence of 2,4-dichlorophenoxyacetic acid and deltamethrin was shown and experimentally proved to be invalid. Three options were proposed for the conditions of the gradient mode of the separate detection of analytes in a mixture.