Izvestiya of Saratov University.
Chemistry. Biology. Ecology
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

фосфатный буферный раствор

Comparison of two types of mobile phases for the determination of B vitamins by thin layer chromatography

Thin-layer chromatography (TLC) has been used to study the infl uence of a number of factors (the nature of the stationary phase, the nature and concentration of the organic solvents and surfactants, the pH of the buff er solution) on the chromatographic characteristics of B vitamins: B1 (thiamine), B6 (pyridoxine), B12 (cyanocobalamin). The stationary phases were plates with polar (Sorbfi l on a polymer (highperformance plates, HTLC) and aluminum substrates), weakly polar (Polyamide-6), and nonpolar (RP-18 on an aluminum substrate) sorbents.

Сравнение двух типов подвижных фаз для определения витаминов группы В методом тонкослойной хроматографии

Методом тонкослойной хроматографии (ТСХ) изучено влияние ряда факторов (природы неподвижной фазы, природы и концентрации органических растворителей и поверхностно-активных веществ, рН буферного раствора) на хроматографические характеристики витаминов группы В: В1 (тиамин), В6 (пиридоксин), В12 (цианокобаламин). В качестве неподвижных фаз (НФ) использовали пластины с полярным (Сорбфил на полимерной (высокоэффективные пластины, ВТСХ) и алюминиевой подложках, слабополярным (Полиамид-6) и неполярным (RP-18 на алюминиевой подложке) сорбентами.

Thin layer chromatography of some statins in aqueous organic mobile phases modified with buff er solutions

The influence of a number of factors on the chromatographic properties of statins (atorvastatin, rosuvastatin, simvastatin) in normal-phase and reverse-phase chromatography modes has been studied by the method of the thin-layer chromatography (TLC) in order to select the most effective analytical systems for the total and separate determination of statins in pharmaceuticals. The study has been performed by ascending TLC on commercial plates with polar, weakly polar, and nonpolar phases.