The article provides information on the current status of populations of 29 plant species from the Orchidaceae family in the south of the Middle Volga region (within the Penza, Samara and Ulyanovsk regions) from Annex II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES): Cephalanthera longifolia (L.) Fritsch, Cephalanthera rubra (L.) Rich., Corallorhiza trifida Chatel., Cypripedium calceolus L., Cypripedium guttatum Sw., Cypripedium macranthos Sw., Cypripedium ventricosum Sw., Dactylorhiza fuchsii (Druce) Soo, Dactylorhiza in