Biology

Fourier Transform Infrared Spectroscopic Analysis of Poly-3-Hydroxybutyrate Accumulation by Azospirillum brasilense Cells at Various Cultivation Periods and Ammonium Concentrations in the Culture Medium

Many bacteria, in response to unfavourable environmental conditions, can synthesise polyesters of the polyhydroxyalkanoate (PHA) class. These biopolymers, accumulating intracellularly in the form of granules, help the bacteria to cope with negative environments and are utilised as reserve sources of carbon and energy. Rhizobacteria of the species Azospirillum brasilense synthesise a single type of PHA, poly-3-hydroxybutyrate (PHB), in response to stress factors.

Analysis of the Systematic Appurtenance of Protozoa from the Soils of the Gorno-Altai High-mountain Plague Focus

Characterization of the species composition of the dominant representatives of Protozoa in natural plague foci is important for the study of certain aspects of ecology and persistence mechanisms of Yersinia pestis. Determination of systematic belonging of amoebas isolated from the soils of the Gorno-Altai high-mountain plague focus in 2016 year was carried out by analyzing nucleotide sequences of ribosomal genes of these protozoans. The analyzed sequences of ribosomal genes were obtained by PCR with specific primers followed by sequencing of PCR products.

Introduction to Culture in vitro of Corn Parthenogenetic Lines

Many modern selection technologies are based on the use of haploid plants as a starting material. The spontaneous frequency of haploid development is very low (0.01–0.1%). In this regard, the creation of plant forms with increased frequency of parthenogenesis is important. The collection of corn lines with an increased frequency of haploid plants in the progeny was created at the Department of Genetics of the Saratov State University. High costs are required for maintain of a plant collections in the field.

Possibility of Determination of Specific Activity of O-Ag in the Production of Cholera Chemical Vaccine by Dot-Analysis

Cholera remains a global threat to public health. Even sporadic cases of importation of the infection cause significant socio-economic damage. In connection with the current situation, the development and improvement of modern safe chemical vaccines against cholera caused by V. cholerae O1 and O139 serogroups plays an important role in reducing the incidence, which is an important and promising direction of scientific research. 

Characterization Surface Glycopolymers from Halophilic Gram-Negative Bacteria Chromohalobacter salexigens 1QL3 and Halomonas ventosae S5(2)

Extracellular and membrane polysaccharides of halophilic bacteria arouse the interest of researchers as promising biopolymers involved in adaptation and maintenance of normal physiology of microorganisms in saline environments that accompany many biotechnological processes. This work aimed at structural characteristic of surface glycopolymers of halophilic Gram-negative bacteria isolated from the salt samples of the lakes Qarun (Egypt) and Elton (Russia) – strains Chromohalobacter salexigens EG1QL3 and Halomonas ventosae RU5S2EL, respectively.

Differentiation of Yersinia pestis Strains of the Main Subspecies of Medieval Biovar by Real-Time PCR Method with Hybridization-Fluorescenсe Registration and SNP Typing

Methods of differentiation and identification of Yersinia pestis strains of medieval biovar by real-time PCR method with hybridization-fluorescent results’ registration and SNP typing method have been developed. Ninety-one Y. pestis strains of medieval biovar from the plague foci of Russia and other CIS countries, received from the State Collection of Pathogenic Bacteria at the Russian Research Anti-Plague Institute “Microbe”, were used in this work. The strains of medieval biovar are represented by four already known phylogenetic lines: 2.MED0, 2.MED1, 2.MED2, 2.MED3.

МЕТОДИЧЕСКИЕ ПОДХОДЫ, МЕТОДЫ И КРИТЕРИИ ОЦЕНКИ АДАПТИВНОСТИ РАСТЕНИЙ

В статье рассматриваются методы оценки пластичности, стабильности и гомеостатичности, обсуждается последовательность оценки экспериментального материала по адаптивным свойствам растений.

РАЗВИТИЕ КАБИНЕТНОЙ СИСТЕМЫ В СОВРЕМЕННОМ ШКОЛЬНОМ БИОЛОГИЧЕСКОМ ОБРАЗОВАНИИ

В статье освещается один из аспектов модернизации российского биологического образования - использование учебно-материальной базы обучения биологии. Приводятся экспериментальные данные по состоянию кабинетов биологии 100 школ Саратовской области. Авторы показывают пути развития кабинетов биологии на современном этапе.

ПОЛОВЫЕ ОСОБЕННОСТИ ЭНЕРГЕТИЧЕСКОГО ОБМЕНА У КРЫС В УСЛОВИЯХ ПОКОЯ И ОСТРОГО СТРЕССА

В статье рассматриваются вопросы, касающиеся возможных причин различной стресс-устойчивости самок и самцов. Было проведено измерение активности сукцинатдегидрогеназы, цитратсинтазы и АТФ-азы, полярографическое исследование дыхания на различных субстратах митохондрий самок и самцов в условиях покоя и острого стресса. Обнаружено, что острый стресс приводит к активации энергетического обмена в митохондриях.

ПОЛОВЫЕ РАЗЛИЧИЯ В РЕАКЦИЯХ СЕРДЕЧНО-СОСУДИСТОЙ СИСТЕМЫ НА МОДУЛЯЦИЮ СИМПАТИЧЕСКОЙ АКТИВНОСТИ У НОРМАЛЬНЫХ И СТРЕССИРОВАННЫХ КРЫС

Изучались половые особенности в реакциях сердечно-сосудистой системы на фармакологическую активацию и торможение адренергических влияний в покое и при стрессе. Показано, что у нормальных и стрессированных самок кардиоваскулярная чувствительность к введению обзидана и адреналина значительно выше, чем у самцов.

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