Obtaining a fluorescently labeled endophytic strain of bacteria Herbaspirillum lusitanum P6-12 for their detection in vivo and in vitro

The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation.

Faunistic complex of mycetophilous beetles (Insecta: Coleoptera) of the xylotrophic fungus Fomes fomentarius (Basidiomyceta) in the territory of the Saratov region

This article describes the characteristics of the beetle fauna – inhabitants of fruiting bodies and the mycelial layer of the true tinder fungus Fomes fomentarius L.: Fr. and are considers some important aspects of the ecology of mycetophilous beetles. Coleopteran population of fruiting bodies of the fungus in five districts of the Saratov region is 63%. Two species from the family Staphylinidae (Gyrophaena joyi Wendeler, 1924 and Scaphisoma boreale Lundblad, 1952) have been recorded on growing and / or spore bearing fruiting bodies.

Evaluation of the secondary structure of poly-γ-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method

An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-γglutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions.

Morphobiology and assessment of the introduction possibilities of Leonurus cardiaca L.

The data on the study of morphometric parameters of one of the most valuable medicinal plants – Leonurus cardiaca L., growing in the collection site of medicinal plants of the Botanical garden of the OSU are presented. The parameters of samples, obtained from the cities of Samara and Kazan were compared . All the studied plants have passed the full cycle of their development with the formation of viable seeds. It is established, that the studied species adapts well to new growing conditions, while the morphometric parameters vary within the middle – very high levels of variability.

Phytochemical composition in the vegetative organs of Hemerocallis hybrida hort. ex Bergmans (Hemerocallidaceae)

The article presents the results of the study of raw materials (sugar, starch) and biologically active substances (pectin substances, catechins, saponins, flavonols, ascorbic acid) in the leaves and rhizomes of Hemerocallis hybrida plants of the Speak to me variety during seasonal development during 2013–2015. The quantitative content of the main groups of substances in underground and aboveground organs during vegetation, flowering and fruiting was determined.

Special features of fruiting in some Clematis L. cultivars from the collection of the Nikita Botanical Gardens

The article presents the results of studying the fruiting in 9 clematis cultivars under the conditions of the southern coast of Crimea. Morphometric characteristics of seeds, their weight and germination capacity are given. Potential and real seed production was assessed. It was found that the studied cultivars are characterized with high potential seed production, on average from 38.1 to 116.9 ovules per fruit, but relatively low real seed production, on average from 1.3 to 8.3 seeds per fruit.

Impact of environmental conditions on the vitality structure of the cenopopulations of the dominant tree tier in the forests of the southern part of the Volga Upland

The vitality structure of cenopulations of woody plants is formed under the influence of ecological, cenotic and anthropogenic influences. The study of the nature and degree of influence of the ecological parameters of the habitat will make it possible to predict the formation of the vitality structure of woody plants. The objects of study are individuals of the generative age state of three species of woody plants: Quercus robur, Tilia cordata, Acer platanoides. Environmental conditions of habitat were assessed by using range ecological scales.

Photocatalytic effect of led radiation (405 nm) and new Al2O3 3D-nanocomposites on the growth of Staphylococcus aureus

The progressive growth of bacterial resistance to antibiotic drugs requires the creation of highly efficient nanomaterials. Aluminum oxide is a stable non-toxic semiconductor material; however, the photocatalytic properties of its modifications in relation to microorganisms are not well understood. In this study, we used new 3D composites of aluminum oxyhydroxide (Al2O3 × nH2O) in three modifications (γ, α and θ), which are a mesh of 150 nm nanofibrils. The assessment of the photocatalytic antibacterial properties of the composites was carried out on the museum strain S. aureus 209 P.

Additions to the «Flora...» by P. F. Maevsky (2014) for the Penza Region

 Records of 94 vascular plant species from the Penza Region published before 2013 are missing from the 11th edition of the «Flora of the Central Part of European Russia» by P. F. Mayevsky (2014). An additional 22 species were discovered in the region recently. At least 29 species known in the Penza Region are completely missing from the «Flora…» – Stellaria subulata Boeber ex D. F. K.

Features of selection of winter wheat on grain quality

The quality of grain means a set of technologico-biochemical, baking and food properties determining the economic value of a grain variety. When choosing source material for selection, the degree of grain preservation in adverse environmental conditions is taken into account as well as the genotypic potential of grain yield and quality.