Biology

Stress signals of water scavenger beetles Berosus frontifoveatus Kuwert, 1888 and Berosus spinosus Steven, 1808 (Coleoptera: Hydrophilidae)

The study of acoustic signals from various representatives of insects, and in particular coleopterans, has a long history. In the Russian-language literature, systematic, faunistic and ecological studies on aquatic beetles, including those on the family Hydrophilidae, are widely known; however, studies on the bioacoustics of the coleopteran group are quite rare. The aim of our study was to study stress signals of two species of Hydrophilidae – Berosus frontifoveatus Kuwert, 1888 and Berosus spinosus Steven, 1808.

The use of games as a method in teaching the basics of chemical toxicology as part of the ecological education of schoolchildren

In today’s modern technogenic world, forming an ecological consciousness, perception and thinking among schoolchildren is a vital task. Knowledge of chemical toxicology is essential for students of all ages to help protect and maintain a healthy environment. Given that toxicology is characterized by an increased level of complexity, the purpose of this work was to develop and apply gaming methods in teaching the basics of chemical toxicology as part of environmental education of schoolchildren.

Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding.

Phylogeography of the scorpion M. сaucasicus (Nordmann 1840) within the range of the genus Mesobuthus (Vachon 1950) (Scorpiones: Buthidae)

Currently, new habitats of scorpions of the genus Mesobuthus have been established on the territory of the republics of Armenia and Dagestan. The aim of the study was to determine the phylogenetic position of scorpions inhabiting the territories of the Republic of Armenia and the Republic of Dagestan. This study contributes to the development of ideas about the zoogeographic distribution of the scorpion fauna in the Palaearctic.

Obtaining a fluorescently labeled endophytic strain of bacteria Herbaspirillum lusitanum P6-12 for their detection in vivo and in vitro

The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation.

Faunistic complex of mycetophilous beetles (Insecta: Coleoptera) of the xylotrophic fungus Fomes fomentarius (Basidiomyceta) in the territory of the Saratov region

This article describes the characteristics of the beetle fauna – inhabitants of fruiting bodies and the mycelial layer of the true tinder fungus Fomes fomentarius L.: Fr. and are considers some important aspects of the ecology of mycetophilous beetles. Coleopteran population of fruiting bodies of the fungus in five districts of the Saratov region is 63%. Two species from the family Staphylinidae (Gyrophaena joyi Wendeler, 1924 and Scaphisoma boreale Lundblad, 1952) have been recorded on growing and / or spore bearing fruiting bodies.

Evaluation of the secondary structure of poly-γ-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method

An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-γglutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions.

Morphobiology and assessment of the introduction possibilities of Leonurus cardiaca L.

The data on the study of morphometric parameters of one of the most valuable medicinal plants – Leonurus cardiaca L., growing in the collection site of medicinal plants of the Botanical garden of the OSU are presented. The parameters of samples, obtained from the cities of Samara and Kazan were compared . All the studied plants have passed the full cycle of their development with the formation of viable seeds. It is established, that the studied species adapts well to new growing conditions, while the morphometric parameters vary within the middle – very high levels of variability.

Phytochemical composition in the vegetative organs of Hemerocallis hybrida hort. ex Bergmans (Hemerocallidaceae)

The article presents the results of the study of raw materials (sugar, starch) and biologically active substances (pectin substances, catechins, saponins, flavonols, ascorbic acid) in the leaves and rhizomes of Hemerocallis hybrida plants of the Speak to me variety during seasonal development during 2013–2015. The quantitative content of the main groups of substances in underground and aboveground organs during vegetation, flowering and fruiting was determined.

Special features of fruiting in some Clematis L. cultivars from the collection of the Nikita Botanical Gardens

The article presents the results of studying the fruiting in 9 clematis cultivars under the conditions of the southern coast of Crimea. Morphometric characteristics of seeds, their weight and germination capacity are given. Potential and real seed production was assessed. It was found that the studied cultivars are characterized with high potential seed production, on average from 38.1 to 116.9 ovules per fruit, but relatively low real seed production, on average from 1.3 to 8.3 seeds per fruit.

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