The study of the resistance of plants obtained as a result of introgressive breeding to various factors, including osmotic stress, is an important stage of breeding work. The objects of research are two varieties of spring soft wheat – Saratovskaya 70 and Saratovskaya 68 and three lines – L3 (C68/ThatcherLr28*4//C68), L4 (C68/ThatcherLr29*4//C68), L12 (C70/ThatcherLr29* 4//C70). PEG was used as a cultivation substrate. The eff ect of osmotic stress on growth was assessed by morphometric parameters, the seedling root availability index, and the seedling root index.