Colchicum

The aim of this work was to identify the degree of tepal bend shape variation in representatives of six Colchicum s.l species: C. laetum L., C. ancyrense B. L. Burtt, C. bulbocodium var. versicolor (Ker Gawl.) K. Perss, C. umbrosum Steven, C. trigynum (Steven ex Adam) Stearn, C. eichleri (Regel) K. Perss. In each of the 53 populations located in the territory of European Russia, 30 plants were studied. The analysis of tepal bend shape variation was carried out using the geometric morphometry method. Principal Component Analysis (PCA) showed that C. laetum and C.

The article presents the results of testing and optimization of the sample preparation technique for fl ow cytometric analysis of two species that have not been previously studied in this aspect: Colchicum bulbocodium subsp. versicolor and C. laetum. We are using optimal LB01 buff er with added sodium thiosulfate, as internal standards we take Petroselinum crispum and Secale cereale. For the fi rst time, the DNA content in living samples of C. bulbocodium subsp. versicolor (2C = 8.049 pg) and C.

The objects of the study were C. laetum L., C. ancyrense B. L. Burtt and C. bulbocodium var. versicolor (Ker Gawl.) K. Pers. The variability of the shape of the bends of the sepals was studied by geometric morphometry. In each of the 30 populations located on the territory of European Russia, 30 samples were examined. To visualize the initial data, principal component analysis (PCA) was used to identify patterns of variability in the shape of the bends of the perianth leafl ets. Only C. bulbocodium subsp. are relatively well separated from each other on this basis.