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Marakaeva A. V., Kosyreva I. V. Visual Colorimetric and Colorimetric Determination of Cefuroxime. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2020, vol. 20, iss. 1, pp. 16-23. DOI:

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Visual Colorimetric and Colorimetric Determination of Cefuroxime


Cefuroxime is a cephalosporin antibiotic with a wide spectrum of antibacterial activity for gram-positive and gram-negative microorganisms. It is in the list of vital and essential drugs for medical use. Currently, spectroscopic, chromatographic, electrochemical methods are used to determine cefuroxime. In current study we developed test agents in the form of indicator papers and powders with an immobilized Fehling reagent for the rapid determination of cefuroxime. Cellulose paper and silica gel were used as a solid matrix to create testing means. The optimal conditions for carrying out the reaction on a solid substrate were selected. Color scales are obtained for visual-colorimetric and colorimetric determination of cefuroxime using indicator papers and powders. Linear dependences of optimal color parameters on antibiotic concentration were constructed. A simple, rapid method for the determination of cefuroxime in drugs was developed. Validation of the developed test methods was carried out by the method of “introduced-found” and the spectrophotometric method. The content of cefuroxime, found using the developed test tools, corresponded to the declared one by the manufacturer (Sr does not exceed 0.14).


1. Arzamascev A. P. Farmacevticheskaya himiya [Pharmaceutical chemistry]. Moscow, GEOTAR-Media Publ., 2004. 640 p. (in Russian).
2. European Directorate for the Quality of Medicines and Healthcare (EDQM). Available at: http// (accessed 18 August 2014).
3. Gosudarstvennaya farmakopeya Rossiyskoy Federatsii [State Pharmacopoeia of the Russian Federation]. 13 ed. Moscow, Federal Electronik Medical Library, 2015, vol. 1, pp. 724–910 (in Russian).
4. Egorov N. S. Osnovy ucheniya ob antibiotikakh [The basics of the doctrine of antibiotics]. Moscow, Nauka Publ., 2004. 524 p.
5. Rasporyazhenie Pravitel’stva RF ot 23 oktyabrya 2017 g. № 2323-r «Ob utverzhdenii perechnya zhiznenno neobhodimykh i vazhneyshikh lekarstvennykh preparatov na 2018 g.» [Decree of the Government of the Russian Federation of October 23, 2017 No. 2323-r “On approval of the list of vital and essential medicines for 2018”]. Available at: (accessed 10 March 2020) (in Russian).
6. Strachunskiy L. S., Kamanin E. I. Antibacterial the rapy of infections in otorhinolaryngology. Russian Medical. Journal, 1998, vol. 6, no. 11, pp. 684‒693 (in Russian).
7. Zubkov M. N. The algorithm for the treatment of acute and chronic infections of the upper and lower respiratory tract. Russian Medical Journal, 2009, no. 2, pp. 123–130 (in Russian).
8. Murillo J. A., Lemus J. M., Garcia L. F. Spectrofl uorimetric Analysis of Cefuroxime in Pharmaceutical Dosage Forms. Journal of Pharmaceutical and Biomedical Analysis, 1994, vol. 12, no. 7, pp. 875‒881. DOI:
9. Kulapina O. I., Mikhailova M. S., Kulapina E. G. Ionometric Determination of Cefuroxime and Cefuroxime Axetil in Biological and Medical Treatment. Izv. Saratov Univ. (N. S.), Ser. Chemistry. Biology. Ecology, 2013, vol. 13, iss. 3, pp. 40‒45 (in Russian).
10. Zivanovic L., Ivanovic I., Solomun L., Zecevic M. Stability Testing of Cefuroxime in Tablets by Micellar Liquid Chromatography. Chromatographia, 2004, vol. 60, no. 1, pp. S61‒S66. DOI:
11. Shelke S., Dongre S., Rathi A., Dhamecha D., Maria S., Dehghan M. H. G. Development and Validation of UV Spectrophotometric Method of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation. Asian Journal of Research in Chemistry, 2009, vol. 2, no. 2, p. 222.
12. Amir S. B., Hossain M. A., Mazid M. A. Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation. Journal of Scientifi c Research, 2014, vol. 6, no. 1, pp. 133‒141. DOI:
13. Ingale P. L., Dalvi S. D., Jadav D. D., Gudi S. V., Patil L. D., Kadam Y. A. Simultaneous Estimation of Cefuroxime Axetil and Potassium Clavulanate-Analytical Method Development and Validation. Der Pharma Chemica, 2013, vol. 5, no. 5, pp. 35‒39.
14. Can N. Ö., Altiokka G., Aboul-Enein H. Y. Determination of Cefuroxime Axetil in Tablets and Biological Fluids Using Liquid Chromatography and Flow Injection Analysis. Analytica Chimica Acta, 2006, vol. 576, no. 2, pp. 246‒252. DOI:
15. Vieira D. C. M., Salgado H. É. R. N. Quantitative Methods for the Identifi cation of Cefuroxime Sodium. Advances in Analytical Chemistry, 2012, vol. 2, no. 5, pp. 67‒73. DOI:
16. Vieira D. C. M., Salgado H. R. N. Comparison of HPLC and UV Spectrophotometric Methods for The Determination of Cefuroxime Sodium in Pharmaceutical Products. Journal of Chromatographic Science, 2011, vol. 49, no. 7, pp. 508‒511. DOI:
17. Feigl F. Spot Tests in Organic Analysis. New York, Elsevier Publishing Company, 1960. 675 р.
18. Lisichkin G. V. Modifi tsirovannye kremnezemy v sorb tsii, katalize i khromatografi i [Modifi ed silicas in sorption, catalysis and chromatography]. Moscow, Khimiya Publ., 1986. 248 p. (in Russian).
19. Reshetnyak E. A., Nikitina N. A., Loginova L. P., Ostrovskaya V. M. Limit of detection in test methods of analysis with visual indication: Affecting factors. Journal of Analytical Chemistry, 2005, vol. 60, no. 10, pp. 1102‒1109 (in Russian).

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