Cite this article as:

Marakaeva A. V., Kosyreva I. V. Test-Determination of Amoxicillin in Medications. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2019, vol. 19, iss. 2, pp. 146-151. DOI: https://doi.org/10.18500/1816-9775-2019-19-2-146-151


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543.068.8
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Russian

Test-Determination of Amoxicillin in Medications

Abstract

Amoxicillin is a semi-synthetic aminopenicillin β-lactam series with a wide spectrum of antibacterial activity against many gram-positive and gram-negative microorganisms. It is included in the list of essential drugs. Test systems for the detection and identification of amoxicillin are missing. We have obtained indicator papers with immobilized ninhydrin and p-dimethylaminobenzaldehyde for the test determination of amoxicillin. The reaction conditions were selected, depending on the temperature and time. Color scales for visual colorimetric and colorimetric determination were constructed. The values of color parameters were determined by RGB, CMYK, HSB color models, the optimal channels were selected for brightness,contrast and sensitivity. The linear dependencies of the chosen color parameters on the concentration of the antibiotic were constructed. Mathematical processing of test tools with the construction of radar charts in the coordinates of the color models R, G, B, C, M, Y, K was carried out. Linear dependencies of the parameters of the area, the perimeter of radar charts on the antibiotic concentration were obtained, allowing a quantitative assessment of the drug content. Based on the studies conducted, a simple, express method for determining the antibiotic in medicinal preparations was developed and tested. The technique was tested on two drugs in tablet form produced by Russian pharmeceutical companies.

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