Cite this article as:

Semyachkina-Glushkovskaya O. V., Gekalyuk A. S., Sindeeva O. A., Abdurashitov A. S. Role of Sulfonylurea Receptor in the Development of Stress-induced Hemorrhagic Cerebral Infarction in Newborn Rats. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2015, vol. 15, iss. 2, pp. 80-85.


Role of Sulfonylurea Receptor in the Development of Stress-induced Hemorrhagic Cerebral Infarction in Newborn Rats


In this article, we develop a model of hemorrhagic infarct of brain in neonatal rats. The results of histological analysis showed that on the next day after exposure to stress sound (120 dB) for 2 hours, there are small focal subcortical hemorrhage, the brain edema, hypoxia and accumulation of blood area of in macro- and microcirculation. Speckle imaging of cerebral circulation revealed hyperperfusion of brain tissue with increase size of the cerebral veins in newborn rats with stress-induced intracranial hemorrhages. These pathological changes in cerebral blood flow accompanied by high expression of sulfonylurea receptors in the brain tissue that is not detected in normal. These results allowed us to conclude that the sulfonylurea receptors may play an important role in the development of hemor- rhagic cerebral infarction in the first days after birth, which is also shown by other researchers on adult rats.


1. Go A. S. Heart disease and stroke statistics ‒ 2013 update : A report from the American Heart Association // Circulation. 2013. Vol. 127. P. 6‒245. 

2. Fox C. K. High critical care usage due to pediatric stroke: Results of a population-based study // Neurology. 2012. Vol. 79. P. 420–427. 

3. Gandhi S. K. Temporal trends in incidence and longterm case fatality of stroke among children from 1994 to 2007 // Neurology. 2012. Vol. 78. P. 1923–1929. 

4. Fullerton H. J. Risk of stroke in children Ethnic and gender disparities // Neurology. 2003. Vol. 61. P. 189–194. 

5. Andersen R. Death : leading causes for 2002 // National Vital Statistics reports. 2005. Vol. 53. P. 17.

6. Lynch J. K. Report of the National Institute of Neurological Disorders and Stroke workshop on perinatal and childhood stroke // Pediatrics. 2002. Vol. 109. P. 116–123. 

7. Beslow L. A. Predictors of outcome in childhood intracerebral hemorrhage : A prospective consecutive cohort study // Stroke. 2010. Vol. 41. P. 313–318. 

8. Fullerton H. J., Zhao S. Risk of stroke in children Ethnic and gender disparities // Neurology. 2003. Vol. 61. P. 189–194. 

9. Ballabh P. Intraventricular hemorrhage in premature infants: mechanism of disease // Pediatr. 2010. Vol. 67. P. 1–8.
10. Giroud M. Cerebrovascular disease in children under 16 years of age in the city of Dijon, France : a study of incidence and clinical features from 1985 to 1993 // J. Clin. Epidemiol. 1995. Vol. 48. P. 1343–1348. 

11. Broderick J. Stroke in children within a major metropolitan area : the surprising importance of intracerebral hemorrhage // J. Child Neurol. 1993. Vol. 8. P. 250–255. 

12. Andersen S. L. Trajectories of brain development : point of vulnerability or window of opportunity // Neurosci. Biobehav. 2003. Vol. 27. P. 3–18. 

13. Brouwer A. J. European perspective on the diagnosis and treatment of posthaemorrhagic ventricular dilatation // Arch. Dis. Child Fetal Neonatal. 2012. Vol. 97. P. 50‒55.
14. Bruno C. J. Hemorrhagic stroke in term and late preterm neonates // Arch Dis Child Fetal Neonatal. 2014. Vol. 99. P. 48‒53.

15. Vohr B.R., Allan W. C. School-age outcomes of very low birth weight infants in the indomethacin intraventricular hemorrhage prevention trial // Pediatrics. 2003. Vol. 98. P. 50‒55. 

16. Kurland D. B. Glibenclamide for the Treatment of Acute CNS Injury. // Pharmaceuticals 2013. Vol. 6. P. 1287‒1303. 

17. Simard J. M. Key role of sulfonylurea receptor 1 in progressive secondary hemorrhage after brain contusion // J. Neurotrauma. 2009. Vol. 26. P. 2257–2267. 

18. Woo S. K. The sulfonylurea receptor 1 (Sur1)-transient receptor potential melastatin 4 (Trpm4) channel // J. Biol. Chem. 2013. Vol. 288. P. 3655–3667. 

19. Simard J. M., Andersen R. Does inhibiting Sur1 complement rt-PA in cerebral ischemia?// N.Y. Acad. Sci. 2012. Vol. 1268. P. 95–107. 

20. Kilbourne M. Key role of sulfonylurea receptor 1 in progressive secondary hemorrhage after brain contusion // J. Neurotrauma. 2009. Vol. 26. P. 2257–2267. 

21. Yamada K. Neuroprotection by KATP channels // J. Mol. Cell. Cardiol. 2005. Vol. 38. P. 945–949.

22. Pavlov A. N., Semyachkina-Glushkovskaya O. V. Multiresolution analysis of pathological changes in cerebral venous dynamics in newborn mice with intracranial hemorrhage : adrenorelated vasorelaxation // Physiol. Measurement. 2014. Vol. 35. P. 1983‒1999. 

23. Semyachkina-Glushkovskaya O. V. The assessment of pathological changes in cerebral blood fl ow in hypertensive rats with stress-induced intracranial hemorrhage using Doppler OCT : particularities of arterial and venous alterations // J. of Photonics and Lasers in Medicine. 2013. Vol. 2, № 2. P. 109‒116.

24. Lychagov V. V. The experimental study of stress-related
pathological changes in cerebral venous blood fl ow in newborn rats assessed by DOCT // J. of Innovative Optical Health Science. 2013. Vol. 3, № 12. P. 1‒9. 

25. Пат. 2506805 РФ. Способ моделирования развития мелкоочаговых мозговых геморрагий в коре головного мозга у новорожденных крыс / Семячкина-Глушковская О. В., Тучин И. А., Заявл. 2012145191 от 24.10.2012; Опубл. 27.01.2014; Бюл. № 3. 

26. Simard J. M., Kilbourne M. Key role of sulfonylurea receptor 1 in progressive secondary hemorrhage after brain contusion // J. Neurotrauma. 2009 Vol. 26. P. 2257–2267. 

27. Thompson E. M. Inhibition of SUR1 Decreases the Vascular Permeability of Cerebral Metastases // J. Neoplasia. 2013. Vol. 15, № 5. P. 535–543. 28. Anderson P. J. Adult outcome of extremely preterm infants // Pediatrics. 2010. Vol. 126. P. 342–351. 29. Zhang J. H. Glibenclamide improves neurological function in neonatal hypoxia-ischemia in rats // J. Brain. 2009. Vol. 1270. P. 131–139. 30. Simard J .M., Castellani R. J. Sulfonylurea receptor 1 in the germinal matrix of premature infants // J. Pediatr. 2008. Vol. 64. P. 648–652.