Cite this article as:

Shutova V. V., Rusyaeva A. B. Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2018, vol. 18, iss. 4, pp. 455-461. DOI: https://doi.org/10.18500/1816-9775-2018-18-4-455-461


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577.32:573.6.086.83:577.114.4/8
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Russian

Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05

Abstract

It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate. Submerged periodic cultivation of the producer on media of different composition was used. The properties of the alginate were controlled by IR spectroscopy.It was shown, that during A. vinelandii cultivation on molasses media, the best substrate concentration for the polysaccharide synthesis is 5% (by sucrose). The IR spectrum of the polysaccharide isolated from the culture liquid and purified alginate was similar. The alginate consists of several fractions with a different molecular weight the largest having a molecular mass of 300 kD.

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