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Verevkina N. N., Medvedeva O. A., Korolev V. A., Shevchenko A. V., Kalutsky P. V. Influence of Probiotic “Bifidumbacterin” on the State of Large Intestine Microbiocenosis, Activity of the Antioxidant System and Processes of Lipid Peroxidation in the Conditions of Gentamycin-Associated Dysbiosis and Anomalous Characteristics of the Magn. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2019, vol. 19, iss. 1, pp. 94-102. DOI: https://doi.org/10.18500/1816-9775-2019-19-1-94-102


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577.115:616.34-008.87-092.9-085
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Russian

Influence of Probiotic “Bifidumbacterin” on the State of Large Intestine Microbiocenosis, Activity of the Antioxidant System and Processes of Lipid Peroxidation in the Conditions of Gentamycin-Associated Dysbiosis and Anomalous Characteristics of the Magn

Abstract

Disturbances in the composition of microbiocenoses are warnings of changes in the physiological status of the organism, associated with the inhibition of immunobiological protection of the organism, its allergization, chronic intoxication, and increased susceptibility to infectious diseases. Some studies confirm the effect of magnetic radiation on abnormal characteristics both on the viability and growth rate of bacteria, and on the activity of various systems, in particular the antioxidant system and the lipid peroxidation system. The aim of our study was to determine the influence of the probiotic “Bifidumbacterin” on the large intestine microbiota and the functional-metabolic activity of the antioxidant system and the processes of lipid peroxidation of colonocytes and blood plasma of experimental animals under conditions of gentamicin-associated dysbiosis weighed down by simultaneous exposure to a magnetic field of increased tension. The study was carried out on 120 mice of the BALB / c line, which were divided into six groups of 20 individuals each. Using experimental animals of all studied groups, we studied the quantitative and qualitative composition of the mucosal microflora of the colon, as well as the content of products of lipid peroxidation and enzymes, of the antioxidant system of colonocytes and blood plasma.The use of the probiotic “Bifidumbacterin”, both in the group “Correction of GMP” and in another experimental group “AMP Correction” had a positive effect on the activity of antioxidant protection enzymes and the content of lipid peroxidation products, but only in colonocyte in the large intestine of mice. Changes in blood plasma were insignificant.

References

1. Bäckhed F., Crawford P. A., O'Donnell D., Gordon J. I. Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor. Proc. Natl. Acad. Sci. USA, 2007, no. 2, pp. 104‒106.

2. Tang M. L. K., Hsiao K. C., Ponsonby A. L., Axelrad C., Pitkin S. Long-term clinical and immunological effects of probiotic and peanut oral immunotherapy after treatment cessation: 4-year follow-up of a randomised, double-blind, placebo-controlled trial. Lancet. Child Adolesc. Health, 2017, no. 1, pp. 97‒105. DOI: https://doi.org/10.1016/S2352-4642(17)30041-X

3. Medvedeva O. A., Kaluckij P. V., Besedin A.V., Zhilyaeva L. V., Medvedeva S. K. Infl uence of lactobacterian on the musine large intensive microfl orae structure and mice blood neutrophils functional-metabolic activity in experimental antibiotic-induced dysbiosis in the condition of the anomalous characteristics of the magnetic fi eld. Man and his Health. Kursk Scientifi c and Practice Bulletin, 2011, no. 1, pp. 10–15 (in Russian).

4. Balcavage Х., Alvager T., Swez J., Goff C., Fox M., Abdullyava S., King M. A mechanism for action of extremely low frequency electromagnetic fi elds on biological systems. Biochem. Biophys. Res. Commun., 1996, no. 2, pp. 374–378. DOI: https://doi.org/10.1006/bbrc.1996.0751

5. Panagopoulos J., Karabarbounis A., Margaritis H. Mechanism for action of electromagnetic fi elds on cells. Biochem. Biophys. Res. Commun., 2002, no.1, pp. 95–102.

6. Grassi С., Ascenzo D. М., Torsello А., Martinotti G., Wolf F., Cittadini A., Azzena B. Effects of 50 Hz electromagnetic fi elds on voltage-gated Ca2 channels and their role in modulation of neuroendocrine cell proliferation and death. Cell Calcium., 2014, no. 4, pp. 307–315. DOI: https://doi.org/10.1016/j.ceca.2003.09.001

7. Oncul S., Cuce E. M., Aksu B., InhanGarip A. Effect of extremely low frequency electromagnetic fi elds on bacterial membrane. Intern. J. Radiat. Biol., 2016, no. 3, pp. 42–49. DOI: https://doi.org/10.3109/09553002.2015.1101500

8. Durackova Z. Some Current Insights into Oxidative Stress. Physiol. Res., 2010, no. 2, pp. 459‒469.

9. Kashkin K. P., Karaeva Z. O. Immunnaya reaktivnost’ organizma i antibioticheskaya terapiya [Body’s immune reactivity and antibiotic therapy]. Leningrad, Medicina Publ., 1984. 200 p. (in Russian).

10. Bogdanova E. A., Nesvizhskij Yu. V, Vorob’ev A. A. A study of pariental gastrointeantinal microfl ora of rats after oral administration of probiotics. Annals of the Russian Academy of Medical Sciences, 2006, no. 2, pp. 6–10 (in Russian). 11. Vorob’ev A. A. Features of the microbiocenosis of parietal mucin of the gastrointestinal tract of rats. Journal of Microbiology, Epidemiology and Immunobiolody, 2005, no. 6, pp. 3–7 (in Russian).

12. Nesvizhskij Yu. V. Microbiocenosis of parietal mucin of the gastrointestinal tract of rats with dysbiosis induced. Journal of Microbiology, Epidemiology and Immunology, 2007, no. 3, pp. 57–60 (in Russian).

13. Makarenko E. V. Complex determination of superoxiddismutase and glutathionereductase activity in erythrocytes of patients with chronic liver diseases. Laboratory Case, 1988, no. 11, pp. 48–50 (in Russian).

14. Korolyuk M. A. Metod of limiting catalase activity. Laboratory Case, 1988, no. 1, pp. 16–19 (in Russian).

15. Rebrova O. Yu. Statisticheskij analiz medicinskih dannyh. Primenenie paketa program Statistica [Statistical analysis of medical data. Application software Statistics]. Moscow, Media Sfera Publ., 2006. 312 p. (in Russian).

16. Medvedeva O. A. Sostoyanie mikrofl ory tolstogo kishechnika cheloveka i zhivotnyh pri vozdejstvii anomal’nogo geomagnitnogo polya [The state of microfl ora of the large intestine of humans and animals when exposed to an anomalos geomagnetic field]. Thesis Diss. Doct. Sci. (Biol.). Orenburg, 2012. 48 p. (in Russian).

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