Cite this article as:

Amelin V. G., Andoralov A. M. Determination of Pesticide Residues in Grapes and Tomatoes by High Resolution Mass-spectrometry. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2015, vol. 15, iss. 2, pp. 39-45.


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UDC: 
636.085.3:577.18:543
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Russian

Determination of Pesticide Residues in Grapes and Tomatoes by High Resolution Mass-spectrometry

Abstract

A method for determining the 20 pesticides (azoxystrobin, boscalid, chlorpyrifos, cyprodinil, diazinon, dimethoate, fluazifop-P-butyl, malathion, metribuzin, penconazole, phosalone, pirimicarb, piri- miphos-methyl, prometryn, propargite, pyraclostrobin, pyrimethanil, triadimenol, triadimefon, trifloxystrobin) in grapes and tomatoes by high performance liquid chromatography with tandem quadrupole – time of flight mass spectrometry detector (HPLC-MS / TOF), using sample preparation QuEChERS. The range determines the content of pesticides by an addition of 10 g of the sample was 0.01–2 mg / kg. The relative standard deviation of the test results does not exceed 14%. The duration of the analysis of 30–40 minutes.

References

1. Мельников Н. Н. Пестициды. Химия, технология и применение. М. : Химия, 1987. 712 с. 

2. ГН 1.2.3111-13. Гигиенические нормативы содержания пестицидов в объектах окружающей среды (перечень). М., 2013. 

3. Гигиенические нормативы содержания действующих веществ пестицидов в объектах окружающей среды, продовольственном сырье, пищевых продуктах. Приложение 15.1. к Разделу 15 Главы II // Единые санитарно-эпидемиологические и гигиенические требования к товарам, подлежащим санитарно-эпидемиологическому надзору (контролю) таможенного союза. URL: www.tsouz.ru 

4. Anastassiades M., Lehotay S. J., Stajnbaher D., Schenck F. J. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and «dispersive solid-phase extraction» for the determination of pesticide residues in produce // J. AOAC Int. 2003.Vol. 86. P. 412‒416. 

5. Lehotay S. J., Son K. A., Kwon H., Koesukwiwat U., Fu W., Mastovska K., Hoh E., Leepipatpiboon N. Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables // J. Chromatogr. A 2010. Vol. 1217. P. 2548‒2560. 

6. Lesueur C., Knittl P., Gartner M., Mentler A., Fuerhacker M. Analysis of 140 pesticides from conventional farming foodstuff samples after extraction with the modified QuECheRS method // Food Contr. 2008. Vol. 19. P. 906–914.
7. Wilkowska A., Biziuk M. Determination of pesticide residues in food matrices using the QuEChERS methodology // Food Chem. 2011. Vol. 125. P. 803–812. 

8. Nguyen T. D., Yu J. E., Lee D. M., Lee G. H. A multiresidue method for the determination of 107 pesticides in cabbage and radish using QuEChERS sample preparation method and gas chromatography mass spectrometry // Food Chem. 2008. Vol. 110. P. 207–213. 

9. Koesukwiwat U., Sanguankaew K., Leepipatpiboon N. Rapid determination of phenoxy acid residues in rice by modifi ed QuEChERS extraction and liquid chromatography- tandem mass spectrometry // Anal. Chim. Acta. 2008. Vol. 626. P. 10–20. 

10. Koesukwiwat U., Lehotaya S. J., Miao S., Leepipatpiboon N. High throughput analysis of 150 pesticides in fruits and vegetables using QuEChERS and lowpressure gas chromatography–time-of-flight mass spectrometry // J. Chromatogr. A 2010. Vol. 1217. P. 6692–6703. 

11. Koesukwiwat U., Lehotay S. J., Mastovska K., Dorweiler K. J., Leepipatpiboon N. Extension of the QuEChERS method for pesticide residues in cereals to fl axseeds, peanuts, and doughs // J. Agric. Food Chem. 2010. Vol. 58. P. 5950–5958. 

12. Fernandez-Alba A. R., Juan F. Garcia-Reyes Large-scale multi-residue methods for pesticides and their degradation products in food by advanced LC-MS // Tr. Anal. Chem. 2008. Vol. 27. P. 973‒990. 

13. Soler C., Pico Y. Recent trends in liquid chromatography-tandem mass spectrometry to determine pesticides and their metabolites in food // Tr. Anal. Chem. 2007. Vol. 26. P. 103‒115. 

14. Thurman E. M., Ferrer I., Fernandez-Alba A. R. Matching unknown empirical formulas to chemical structure using LC/MS TOF accurate mass and database searching: example of unknown pesticides on tomato skins // J. Chromatogr. A. 2005. Vol. 1067. P. 127–134. 

15. Mezcua M., Malato O., Garcia-Reyes J. F., Molina-Diaz A., Fernandez-Alba A. R. Accurate-Mass Databases for Comprehensive Screening of Pesticide Residues in Food by Fast Liquid Chromatography Time-of-Flight Mass Spectrometry // Anal. Chem. 2009. Vol. 81. P. 913–929.
16. Ferrer I., Thurman E. M., Fernandez-Alba A. R. Quantitation and Accurate Mass Analysis of Pesticides in Vegetables by LC/TOF-MS // Anal. Chem. 2005.
Vol. 77. P. 818‒2825. 

17. Lacina O., Urbanova J., Poustka J., Hajslova J. Identifi cation/quantifi cation of multiple pesticide residues in food plants by ultra-high-performance liquid
chromato graphy-time-of-fl ight mass spectrometry // J. Chromatogr. A. 2010. Vol. 1217. P. 648–659. 

18. Ferrer I., Garcia-Reyes J. F., Fernandez-Alba A. Identification and quantitation of pesticides in vegetables by liquid chromatography time-of-fl ight mass spectrometry // Tr. Anal. Chem. 2005. Vol. 24. P. 671‒682.