Cite this article as:

Sharavin D. Y., Solomennyi А. P. Conserved and Variable Regions of the Methanol Dehydrogenase Gene Among Halotolerant Representatives of Methylophaga Genus. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2017, vol. 17, iss. 4, pp. 452-457. DOI: https://doi.org/10.18500/1816-9775-2017-17-4-452-457


Heading: 
UDC: 
579.64: 579.84
Language: 
Russian

Conserved and Variable Regions of the Methanol Dehydrogenase Gene Among Halotolerant Representatives of Methylophaga Genus

Abstract

We investigated conserved and variable regions of the mxaF gene and amino acid sequence in methylotrophic strain M1K isolated from saline soil rhizosphere and belonging to Methylophaga genus. The significant heterogeneity was revealed in methano- and methylotrophic bacteria by method of Minimum Evolution. The ability of strain to influence indole-3-acetic acid synthesis from L-tryptophan and halotolerance are the arguments in favor of application in new agrotechnology.

References
  1. Kutschera U. Plant-associated methylobacteria as coevolved phytosymbionts // Plant Signaling & Behavior. 2007. Vol. 2. P. 74–78.
  2. Фёдоров Д. Н., Доронина Н. В., Троценко Ю. А. Фитосимбиоз аэробных метилобактерий : новые факты и гипотезы // Микробиология. 2011. Т. 80, № 4. С. 435–446.
  3. Троценко Ю. А., Доронина Н. В., Торгонская М. Л. Аэробные метилобактерии. Пущино : ОНТИ ПНЦ РАН, 2010. 325 с.
  4. Lau E., Fisher M. C., Steudler P. A., Cavanaugh C. M. The methanol dehydrogenase gene mxaF, as a functional phylogenetic marker for proteobacterial methanotrophs in natural environments // PLOS ONE. 2013. Vol. 8. P. e56993.
  5. Janvier M., Frehel C., Grimont F., Gasser F. Methylophaga marina gen. nov., sp. nov. and Methylophaga thalassica sp. nov., marine methylotrophs // Intern. J. Syst. Bacteriol. 1985. Vol. 35. P. 131–139.
  6. Villeneuve C., Martineau C., Maufferey F., Villemur R. Methylophaga nitratireducenticrescens sp. nov. and Methylophaga frappieri sp. nov., isolated from the biofi lm of the methanol-fed denitrifi cation system treating the seawater at the Montreal Biodome // Intern. J. Syst. Evol. Micr. 2013. Vol. 63. P. 2216–2222.
  7. Gordon S. A., Weber R. P. Colorimetric estimation of indole-acetic acid // Plant Physiol. 1951. Vol. 26. P. 192–195.
  8. Lane D. J. 16S/23S rRNA sequencing // Nucleic acid techniques in bacterial systematic / eds. E. Stackebrandt, M. Goodfellow. N.Y. : Academic Press, 1991. P. 115–167.
  9. Gogleva A. A., Kaparullina E. N., Doronina N. V., Trotsenko Y. A. Methylobacillus arboreus sp.nov. and Methylobacillus gramineus sp.nov., novel nonpigmented obligate methylotrophic bacteria associated with plants // Syst. Appl. Microbiol. 2011. Vol. 34. P. 477–481.
  10. Doronina N. V., Darmaeva T. D., Trotsenko Y. A. Methylophaga alcalica sp. nov., a novel alkaliphilic and moderately halophilic, obligately methylotrophic bacterium from an East Mongolian saline soda lake // Intern. J. Syst. Evol. Micr. 2003. Vol. 53. P. 223–229.
  11. Kim H. G., Doronina N. V., Trotsenko Y. A., Kim S. W. Methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium // Intern. J. Syst. Evol. Micr. 2007. Vol. 57. P. 2096– 2101.
  12. Antony C. P., Doronina N. V., Boden R., Trotsenko Y. A., Shouche Y. S., Murrell J. C. Methylophaga lonarensis sp. nov., a moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater // Intern. J. Syst. Evol. Micr. 2012. Vol. 62. P. 1613– 1618.
  13. Доронина Н. В., Ли Ц. Д., Иванова Е. Г., Троценко Ю. А. Methylophaga murata sp. nov. – галоалкалофильный аэробный метилотроф из разрушающегося мрамора // Микробиология. 2005. Т. 74, № 4. С. 511–519. 
  14. 14. Jeong J. H., Kim S. W., Yoon S. M., Park J. K., Lee J. S. Characterization of the conserved region of the mxaF gene that encodes the large subunit of the methanol dehydrogenase from a marine methylotrophic bacterium // Mol. Cells. 2002. Vol. 13, № 3. P. 369–376.
  15. Graziano G., Merlino A. Molecular bases of protein halotolerance // Biochim. et Biophys. Acta. 2014. Vol. 1844, № 4. P. 850–858.
Short text (in English): 
Full text (in Russian):