Cite this article as:

Тимофеева С. Н., Юдакова О. И., Эльконин Л. А. Breaking Physical Dormancy of the Laburnum anagyroides Seeds by in vivo and in vitro Conditions. Izvestiya of Saratov University. New series. Series: Chemistry. Biology. Ecology, 2017, vol. 17, iss. 1, pp. 30-35. DOI: https://doi.org/10.18500/1816-9775-2017-17-1-30-35


Heading: 
UDC: 
581.6:601
Language: 
Russian

Breaking Physical Dormancy of the Laburnum anagyroides Seeds by in vivo and in vitro Conditions

Abstract

Laburnum anagyroides Medik. is a popular ornamental tree native to the Mediterranean region. Its seeds are characterized of a physical dormancy, complicating seed reproduction. The present study was conducted to identify the seed dormancy breaking treatments to improve seed germination. The various stratification techniques (cold and warm treatments, alternate temperature regime) were tested. Simultaneously, the seeds were germinated in soil and on different nutrient media in vitro. The hot water treatment for 20 minutes and followed cultivating on the MS medium, supplemented with 0.5 mg/l BAP, was a most effective (77.7–83.8% of germinate seeds).

References

1. Baskin C. C., Baskin J. M. Seeds : Ecology, biogeography, and evolution of dormancy and germination (2nd ed.). San Diego : Academic Press, 2014. 1600 p.

2. Grime J. P., Mason G., Curtis A. V., Rodman J., Band S. R., Mowforth M.A.G., Neal A. M., Shaw S. A comparative study of germination characteristics in a local flora // J. of Ecology. 1981. Vol. 69. P. 1017– 1059.

3. Hu X. W., Wu Y. P., Wang Y. R. Different requirements for physical dormancy release in two populations of Sophora alopecuroides relation to burial depth // Ecol. Res. 2009. Vol. 24. P.1051–1056.

4. Renzi J. P., Chantre G. R., Cantamutto M. A. Effect of water availability and seed source on physical dormancy break of Vicia villosa ssp. villosa // Seed Scien. Res. 2016. Vol. 26, № 3. P. 254–263.

5. Schmidt L. H. Tropical Forest Seed. Berlin : SpringerVerlag, 2007. 408 p.

6. Николаева М. Г., Разумова М. В., Гладкова В. Н. Справочник по проращиванию покоящихся семян. Л. : Наука. Ленингр. отд-ние, 1985. 245 с.

7. Baskin J. M., Baskin C. C., Li X. Taxonomy, anatomy and evolution of physical dormancy in seeds // Plant Species Biology. 2000. Vol. 15. P. 139–152.

8. Baskin J. M., Baskin C. C. Classification, biogeography, and phylogenetic relationships of seed dormancy // Seed conservation : turning science into practice. Kew, UK: The Royal Botanic Gardens Press, 2004. P. 517–544.

9. Jaganathana K. G., Yuleb K., Liu B. On the evolutionary and ecological value of breaking physicaldormancy by endozoochory // Perspectives in Plant Ecology, Evolution and Systematics. 2016. Vol. 22. P. 11–22.

10. Ray A., Bhattacharya S. An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride // Plant Cell Tissue Organ Cult. 2008. Vol. 92. P. 315–319.

11. Parveen S., Shahzad A., Saema S. In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance // Agrofor Syst. 2010. Vol. 80. Р. 109–116.

12. Barekat T., Otroshy M., Samsam-Zadeh B., Sadrarhami A., Mokhtari A. A novel approach for breaking seed dormancy and germination in Viola odorata (A medicinal plant) // J. of Novel Appl. Scien. Available. 2013. Vol. 2, № 10. P. 513–516.

13. Dahanayake N. Application of Seed treatments to increase germinability of cardamom (Elettaria cardamomum) seeds under in vitro conditions // Sabaragamuwa Univ. J. 2014. Vol. 13, № 2. P. 23–29.

14. Rego M. M., Rego E. R., Nattrodt L. P. U., Barroso P. A., Finger F. L., Otoni W. C. Evaluation of different methods to overcome in vitro seed dormancy from yellow passion fruit // Afric. J. of Biotechnol. 2014. Vol. 13, № 36. P. 3657–3665.

15. Hewood V. H. Flowering plants of the world. Batsford : BT Londres, 1993. 336 p.

16. Hartmann H. T., Kester D. E., Davies F. T., Geneve R. Plant propagation : principles and practices. Berlin ; Heidelberg : Springer-Verlag, 2010. 864 p.

17. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures // Physiol. Plant. 1962. Vol. 15. P. 473–497.

18. Lloyd G., McCown B. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture // Int'l. Plant Prop. Soc. Comb. Proc. 1980. Vol. 30. P. 421–427.

19. Moreno-Casasola P., Grime J. P., Martinez L. A comparative study of the effects of fluctuations in temperature and moisture supply on hard coat dormancy in seeds of coastal tropical legumes in Mexico // J. of Tropic. Ecology. 1994. Vol. 10. P. 67–86.

20. Horimoto T., Koshioka M., Kubota S., Mander L. N., Hirai N., Ishida N., Suh J. K., Lee A. K., Roh M. S. Effect of warm and cold stratification on ′H-NMR profiles, endogenous gibberellins and abscisic asid in Styrax japonicus seeds // Hort. Environ. Biotechnol. 2011. Vol. 52, № 3. P. 233–239.

Short text (in English): 
Full text (in Russian):