Gene Expression of Specific Factors Transcription Exp1 during Morphogenesis Xylotrophic Basidiomycete Lentinus Edodes


It was established that gene expression exp1, encoding a specific transcription factor was activated during transition the vegetative to the generative stage of development in cultivating xylotrophic basidiomycete Lentinus edodes (shiitake) on wood substrate. Especially this is characteristic from stage previous of fruiting and connected with the formation of brown mycelial mat. A study showed that exp1 gene transcriptional activity increased 10-fold during formation of the morphological structure, while drop in activity of the gene was marked for primordial and fruiting bodies. In cultivation on artificial nutrient media exp1 gene activity in all stages of development was suppressed, whereas when grown in vivo on the wood substrate level of activity above that a specific transcription factor 15–150-fold, depending of the stage of morphogenesis. Exp1 increased activity as maturation basidum. In old fruit bodies of transcriptional activity of the gene was 4–5 of times larger than that of the primordial. In all likelihood, a specific transcription factor exp1 from shiitake may be involved in the aging process. Thus, the activation of the gene may serve as marker exp1 readiness xylotrophic fungi to fruit, as well as an indicator of the adequacy of the culture conditions.


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